Rapid Rubber Extraction and NMR Spectroscopy of Rubber, Extracted from the Endemic Species Scorzonera Tau-Saghyz

  • K. K. Boguspaev Scientific Research Institute of Biology and Biotechnology Problems, 71 al-Farabi ave., Almaty, Kazakhstan
  • S. K. Turasheva Scientific Research Institute of Biology and Biotechnology Problems, 71 al-Farabi ave., Almaty, Kazakhstan; al-Farabi Kazakh National University, Department of Biotechnology, 71 al-Farabi ave., Almaty, Kazakhstan
  • T. M. Seilkhanov Sh. Ualikhanov Kokshetau State University, 76 Abay ave., Kokshetau, Kazakhstan
  • D. G. Faleev Scientific Research Institute of Biology and Biotechnology Problems, 71 al-Farabi ave., Almaty, Kazakhstan
  • M. S. Mutalkhanov al-Farabi Kazakh National University, Department of Biotechnology, 71 al-Farabi ave., Almaty, Kazakhstan
  • V. H. Portnoy Tel Aviv University, The Institute for Cereal Crops Improvement, 205, 69978 , Tel Aviv, Israel
Keywords: Scorzonera tau-saghyz, natural rubber, extraction, regeneration

Abstract

Scorzonera tau-saghyz Lipsch. et G.G. Bosse is an endemic rubber producing plant, growing in mountain regions in South Kazakhstan. The rubber content in plants and the quality of biopolymer has an important impact on industrial rubber production. The results of this study showed that the amount of rubber in S. tau-saghyz roots fluctuates between 7.74% and 38.75%. The amount of synthesized and deposited rubber biopolymer particles depends on various factors such as physiological age of plant, origin, temperature, moisture and environmental conditions. We optimized the extraction method of natural rubber by using n-hexane as a solvent for direct extraction. This method allows extracting the maximum amount of rubber from 3‒4-year-old plants. NMR results show structural links of natural isoprene rubber in the root extract sample. There is a clear relationship between methyl, methine and methylene protons which corresponds to isoprene rubber structure. The samples having strongly marked singlets that are inherent for rubber functional groups confirms the stereospecific structure of rubber. Good solubility of the root extract in deuterated chloroform can characterize the low molecular weight of the polymer. NMR characterization of rubber, extracted from S. tau-saghyz roots, is reported for the first time. Regeneration in vitro provides an important opportunity for endemic preservation by rapidly increasing the number of plants. The best regeneration of adventitious shoots was obtained on MS medium containing 5.5 μM kinetin and 0.5 μM NAA. The plants were successfully acclimatized in a glasshouse with 75% of S. tau-saghyz plantlets, respectively surviving after transfer to ex vitro conditions.

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Published
2020-03-26
How to Cite
[1]
K. Boguspaev, S. Turasheva, T. Seilkhanov, D. Faleev, M. Mutalkhanov, and V. Portnoy, “Rapid Rubber Extraction and NMR Spectroscopy of Rubber, Extracted from the Endemic Species Scorzonera Tau-Saghyz”, Eurasian Chem. Tech. J., vol. 22, no. 1, pp. 59-68, Mar. 2020.
Section
Articles