Catalase Immobilized on Poly(Acrylic Acid-co-Vinyl Alcohol)

Authors

  • Marcel Popa Department of Macromolecules,“Gh. Asachi” Technical University of Iasi, Bd. D.Mangeron, nr. 71 A, 6600 Iasi, Romania
  • Nicu Bajan ICPCH Research Institut, Mihai Sebastian, nr. 24, Braila, Romania
  • Valeriu Sunel Department of Organic Chemistry, “Al.I.Cuza” University of Iasi, Bd. Carol, nr.11, 6600 Iasi, Romania
  • Mihai Daranga Department of Macromolecules,“Gh. Asachi” Technical University of Iasi, Bd. D.Mangeron, nr. 71 A, 6600 Iasi, Romania

DOI:

https://doi.org/10.18321/ectj535

Abstract

The paper studies the reaction of catalase’s immobilization on a synthetic polymer (copolymer of the acrylic acid with vinyl alcohol), through amidation of enzyme’s terminal amine groups with the lateral carboxylic group of the macromolecular support, as activated by dicycloxexyl carbodiimide. The support, possessing the properties of a hydrogel, has been synthesized through copolymerization of the acrylic acid with vinyl acetate, followed by hydrolysis of the acetate groups to the hydroxylic groups. The influence of some reaction parameters (such as the activator/support and enzyme/support ratios, duration) on the efficiency of the enzyme’s coupling was studied. The coupling reactions were realized conformely to a centered, rotator, composed, second order experimental program, which permitted the establishment of the conditions necessary for obtaining a coupling product containing the highest amount of immobilized enzyme. Kinetic study of the reaction catalyzed by the coupling products has indirectly evidenced enzyme’s immobilization to the macromolecular support, and also, a sufficiently high catalytic activity of the enzymatic preparation obtained.

References

(1). Chibata, I., Immobilized Enzymes, John Wiley, N.Y., 1978.

(2). Braun, G.B., Manecke, G. and Wingard, L.B., Enzyme Engineering, vol. 4, Plenum Press, N.Y., 1978.

(3). Dumitriu, S., Popa, M. and Dumitriu, M., J.Biactive and. Compaible. Polymers, 3 – July, 243 (1988).

(4). Weatersby, P.K., Horbett, T.A. and Hoffman, A.S., J. Bioengineering, 1, 381 (1977).

(5). Maeda, H. and Suzuki, H., Biotehnology and Bioengineerig, 15, 403 (1973).

(6). Solomon, B. and Levin, Y., Biotehnology and Bioengineerig, 16, 1161 (1974).

(7). Watanebe, T., Fujimura, M., Mori, T., Tosa, T.and Chibata, I., J.Applied Biochemistry, 7, 28(1979).

(8). Watanabe, T., Mori, T., Tosa, T. and Chibata, I., Biotehnology and Bioengineerig, 21, 477(1979).

(9). Bayhan, M. and Tuncel, A., J. Applied Polymer Science, 67, 6, 1127 (1998).

(10). Sakai, Y., Oishi, A. and Takahashi, F., Biotehnology and Bioengineerig, 62, 3, 363 (1999).

(11). Uludag, H., Norrie, B., Kousinioris, N. and Gao,T., Biotehnology and Bioengineerig,73, 6, 510(2001).

(12). Cochran, W.O. and Cox, G.M., Experimental Designs, J.Wiley and Sons Ltd., Academic Press,New York, 335, 1968.

(13). Thomas, D and Broun, G., Biochimie, 55, 975 (1973).

(14). Johansson, A.C. and Mossbach, Z., Biochim. Biophys.Acta, 370, 348 (1974).

Downloads

Published

2002-08-20

How to Cite

Popa, M., Bajan, N., Sunel, V., & Daranga, M. (2002). Catalase Immobilized on Poly(Acrylic Acid-co-Vinyl Alcohol). Eurasian Chemico-Technological Journal, 4(3), 199–206. https://doi.org/10.18321/ectj535

Issue

Vol. 4 No. 3 (2002)

Section

Articles

License

You are free to: Share — copy and redistribute the material in any medium or format. Adapt — remix, transform, and build upon the material for any purpose, even commercially.

Eurasian Chemico-Technological Journal applies a Creative Commons Attribution 4.0 International License to articles and other works we publish.

Subject to the acceptance of the Article for publication in the Eurasian Chemico-Technological Journal, the Author(s) agrees to grant Eurasian Chemico-Technological Journal permission to publish the unpublished and original Article and all associated supplemental material under the Creative Commons Attribution 4.0 International license (CC BY 4.0).

Further distribution of this work must maintain attribution to the author(s) and the published article’s title, journal citation, and DOI.