Biodegradation of Oil Hydrocarbons by Soil Microflora Activated with Photoluminescence Films

Authors

  • L.I. Svarovskaya Institute of Petroleum Chemistry, Siberian Branch of the Russian Academy of Sciences 3, Academichesky Ave., Tomsk, Russia
  • L.K. Altunina Institute of Petroleum Chemistry, Siberian Branch of the Russian Academy of Sciences 3, Academichesky Ave., Tomsk, Russia
  • D.A. Filatov Institute of Petroleum Chemistry, Siberian Branch of the Russian Academy of Sciences 3, Academichesky Ave., Tomsk, Russia

DOI:

https://doi.org/10.18321/ectj438

Abstract

Stimulating effect of polyethylene photoluminescence films on the growth of autochthonous microflora population in oil-polluted soils has been determined under the laboratory conditions, where oil concentration in the soil was 50 g/kg. The increase in number of basic groups of microflora was accompanied with the increased activity of the enzymes, which catalyzed oxidizing processes. At the end of the experiment, on the 45-th day, oil content decreased to 15 g/kg. The analysis of the residual hydrocarbons by IR-spectroscopy has revealed absorption bands in the regions of 1710 cm–1 and 1600 cm–1. At the same time spectral coefficients С1, С2, А1 and А2 increased 1.5-3 times, while С3 and А3 decreased 1.5-2.5 times indicating oxidation processes of oil hydrocarbons. Chromatographic analysis proved the intensity of hydrocarbon biodegradation. Hydrocarbons С11-С14 completely eliminated in the test samples and the concentration of hydrocarbons with a high molecular weight decreased by 70-80%. At the same time hydrocarbon biodegradation index, determined as the ratio of the sum of isoprenoids (pristine + phytane) to the sum of n-alkanes (С17 + С18),
increased 5-6 times due to the use of photoluminescence films.

References

1. Rozanova, E.P. (1967) The use of hydrocarbons by microorganisms. Uspekhi microbiologii. No 4. pp. 61-93.

2. Atlas R.M., Bartha R. Biodegradation of petroleum in seawater at low temperatures. Canad. J. Microbiol. 1972. V. 18. pp. 1851-1855.

3. Wagner M. Microbial enthancement of oil recovery from carbonate reservoirs with complex formation characteristics. Dev. Petrol. Sci. 1991. V. 31. pp. 387-398.

4. Svarovskaya L.I., Altunina L.K Biodestruction of Petroleum Hydrocarbons. Eurasian Chemico-Technological Journal, 2001. V.3. No 2. pp.125-130.

5. Svarovskaya L.I., Altunina L.K. The Role of Reservoir Microflora in the Process of Oil Displacement with Combined Physico-Chemical and Microbiological Method. Eurasian Chemico-Technological Journal, 2001. V. 3. No 2. pp. 125-130.

6. Khaziev F.H., Fatkhiev F.F. (1981) Change in biological processes proceeding in soils at oil contamination and activation of oil decomposition. Agrokhimiya. V. 1. No 10. pp. 102-111.

7. Kusnetsov S.I., Leplianin G.V. "Polisvetan", a high performance material for cladding grinhouses. Plasticulture, 1989. V.3. No 2. pp. 66-73.

8. RF patent No 2178429 of 20.01.2002. Polymer composition. Minich A.S., Raida V.S., Mayer E.A.

9. Minich A.S., Minich I.B., Raida V.S., Karnachuk R.A., Tolstikov G.A. (2003) Biological testing of light-corrective films at growing of white cabbage in greenhouses. Selskokhozyaistvennaya biologiya. No 3. pp. 112-115.

10. Zvyagintsev D.G. (1991) Methods of soil microbiology and biochemistry. M.: Publishing House MGU. p. 231.

11. Kireeva N.A., Vodopyanov V.V., Miftakhova A.M. (2001) Biological activity of oil-contaminated soils. Ufa. Publishing House "Gilem". p. 330.

12. Bolshakov G.F. (1986) Infrared spectra of saturated hydrocarbons. Part 1. Alkanes. Novosibirsk: Nauka.
pp. 3-32.

13. Laboratory manual on chromatographic and adjacent methods (1982). Edited by O. Mikesha. М.: Mir. Part II. P. 381.

14. Rylkova A.V. (1977) Methods for the analysis of organic matter in oil and gas rocks. Tyumen. ZapSibNIGNI. pp. 16-21.

15. Filatov D.A., Svarovskaya L.I., Altunina L.K. Stimulation of oxygenase activity of oil-oxidizing microflora under the conditions of greenhouses with light-corrective films. Proc. of the VI-th Intern conf. "Oil and Gas Chemistry", September 5-9, 2006. Tomsk. Russia. pp. 279-281.

16. Svarovskaya L.I., Altunina L.K. Activity of soil microflora under the conditions of oil pollutions. Biotekhnologia. Theoretical and Scientific-Practical Journal. 2004. No 3. pp. 63-69.

Downloads

Published

2008-01-15

How to Cite

Svarovskaya, L., Altunina, L., & Filatov, D. (2008). Biodegradation of Oil Hydrocarbons by Soil Microflora Activated with Photoluminescence Films. Eurasian Chemico-Technological Journal, 10(1), 61–66. https://doi.org/10.18321/ectj438

Issue

Vol. 10 No. 1 (2008)

Section

Articles

License

You are free to: Share — copy and redistribute the material in any medium or format. Adapt — remix, transform, and build upon the material for any purpose, even commercially.

Eurasian Chemico-Technological Journal applies a Creative Commons Attribution 4.0 International License to articles and other works we publish.

Subject to the acceptance of the Article for publication in the Eurasian Chemico-Technological Journal, the Author(s) agrees to grant Eurasian Chemico-Technological Journal permission to publish the unpublished and original Article and all associated supplemental material under the Creative Commons Attribution 4.0 International license (CC BY 4.0).

Further distribution of this work must maintain attribution to the author(s) and the published article’s title, journal citation, and DOI.