Influence of Phytohormones on Monosaccharide Composition of Polysaccharides from Wheat Suspension Culture

  • N. K. Bishimbayeva The Institute of Plant Biology and Biotechnology, 45 Timiryazev str., Almaty, Kazakhstan
  • A. S. Murtazina The Institute of Plant Biology and Biotechnology, 45 Timiryazev str., Almaty, Kazakhstan
  • G. J. McDougall James Hutton Institute, Invengowrie, Dundee, DD2, 5DA, Scotland, United Kingdom
Keywords: polysaccharides, wheat cell culture, monosaccharide composition, abscisic acid, 2,4-dichlorophenoxyacetic acid, extracellular substances

Abstract

Plant polysaccharides with technical and physiologic traits attract researchers by their high physiological activity in regulation of the growth, development and protective reactions. Cell cultures allow to regulate chemical composition of synthesized substances by changing media composition and are widely used to enhance or change the biosynthesis of metabolites. The aim of this study was to investigate the influence of phytohormones 2,4-dichlorphenoxyacetic acid (2,4 –D) and abscisic acid (ABA) of culture medium on chemical composition of polysaccharides (PS), extracted from cells and extracellular liquid of wheat suspension culture. It was shown for the medium with ABA that monosaccharide composition of extracellular PS mainly represented by glucose (87%), whereas PS isolated from cells were rich for xylose and glucuronic acid. Monosaccharide composition of extracellular PS from media with 2,4-D showed 6-fold increase of arabinose, 8-fold ‒ of galactose, 5-fold ‒ of xylose and glucuronic acid, compared to extracellular PS from ABA medium. Composition of cellular PS from media with 2,4-D were mainly similar to ABA and differed by the increased amount of mannose (3-fold), and galacturonic acid (2,5-fold). Thus, regulative effect of the use of two different types of phytohormones was demonstrated on the biosynthesis of variously composed polysaccharides.

 

References

[1]. F. Goubet, C. Morvan, Plant Cell Physiol. 35 (5) (1994) 719‒727. Crossref

[2]. E.A. Gunter, Yu.S. Ovodov, Khimija Rastitel’nogo Syr’ja [Chemistry of plant raw materials] 2 (2001) 57‒62 (in Russian).

[3]. M. Ochoa-Villarreal, E. Aispuro-Hernández, M.A. Martínez-Téllez and I. Vargas-Arispuro (Ed. Ailton De Souza Gomes), Polymerization. Chapter 4 (2012) 63‒74. Crossref

[4]. H. Waggner, H. Stuppner, W. Schafer, M. Zenk, Phytochemistry 27 (1988) 119‒126. Crossref

[5]. J. Roesler, A. Emmensuffer, C. Steinmiller, B. Luetting, H. Wagner, M.L. Lohman-Matthes, Int. J. Immunopharmacol. 13 (1991) 931‒941. Crossref

[6]. U.S. Ovodov, Bioorganicheskaya Khimiya, [Russian Journal of Bioorganic Chemistry] 24 (1998) 483‒501 (in Russian).

[7]. I.A. Sartbayeva, E.A. Gunter, N.K. Bishimbayeva, Polysaccharide composition of wheat cell culture. Plant physiology, biochemistry, genetic and breeding research investigations in Kazakhstan, Digest of articles. Ed. F.A. Polymbetova, B.A. Sarsenbayev, Almaty p. 235–241 (in Kazakh). ISBN: 9965-852-57 x

[8]. N.K. Bishimbayeva, А.K. Amirova, A.S. Murtazina, I.A. Sartbayeva, Е.V. Rakhimova, I.R. Rakhimbaev. Study the cells with signs of PCD in cereals embryogenic tissue culture. Proc. of Meeting of SEB. – Czech Republic. Praque, 2010. Р. 137.

[9]. N.K. Bishimbayeva, A.K. Amirova, A.S. Murtazina, G.J. McDougall, I.R. Rakhimbayev, The Biological activity of extracellular polysaccharides of wheat cell suspension culture. Plant physiology, biochemistry, genetic and breeding research investigations in Kazakhstan, Digest of articles. Ed. F.A. Polymbetova, B.A. Sarsenbayev, Almaty, 2010, p. 103‒111 (in Russian) ISBN: 9965-852-57x

[10]. Sarah A. Wilson and Susan C. Roberts, Plant Biotechnol. J. 10 (2012) 249–268. Crossref

[11]. T. Murashige, F.A. Scoog, Physiol. Plant. 13 (1962) 473–497. Crossref

[12]. Ni Xiu-zhen, Wang Bing-qing, Zhi Yuan, Wei Ning-ning, Zhang Xu, Li Shan-shan, Tai Gui-hua, Zhou Yi-fa, Zhao Ji-ming, Chem. Res. Chinese Universities 26 (2010) 230–234.

[13]. M. Dubois, K.A. Gilles, J.K. Hamilton, P.A. Rebers, F. Smith, Anal. Chem. 28 (3) (1956) 350–356. Crossref

[14]. N.K. Bishimbayeva, I.A. Sartbayeva, A.S. Murtazina, E.A. Gunter, International Journal of Biology and Chemistry 8 (2) (2015) 13–17.

[15]. H.J. Hwang, S.W. Kim, J.W. Choi, J.W. Yun, Enzyme Microb. Technol. 33 (2003) 309–319. Crossref

[16]. Xia Guo, Xiang Zou, Min Sun, Bioproc. Biosyst. Eng. 32 (5) (2009) 701–707. Crossref

[17]. K. Otsuji, Y. Honda, Y. Sugimura, A. Takei, T. Tejima, Plant Tissue Culture Lett. 12 (1995) 8–15. Crossref

[18]. R. González-Lamothe, M. El Oirdi, N. Brisson, K. Bouarab, Plant Cell 24 (2012) 762–777. Crossref

[19]. O. Windram, P. Madhou, S. McHattie, C. Hill, R. Hickman, E. Cooke, D.J. Jenkins, C.A. Penfold, L. Baxter, E. Breeze, S.J. Kiddle, J. Rhodes, S. Atwell, D.J. Kliebenstein, Y.S. Kim, O. Stegle, K. Borgwardt, C.J. Zhang, A. Tabrett, R. Legaie, J. Moore, B. Finkenstadt, D.L. Wild, A. Mead, D. Rand, J. Beynon, S. Ott, V. Buchanan-Wollaston, K.J. Denby, Plant Cell. 24 (2012) 3530–3557. Crossref

[20]. P.J. Talboys, H.M. Zhang, J.P. Knox, New Phytol. 190 (2011) 618–626. Crossref

[21]. H. Iwai, T. Ishii, S. Satoh, Planta 213 (2001) 907–915. Crossref

[22]. Y. Verhertbruggen, S.E. Marcus, J.S. Chen, J.P. Knox, Plant Cell Physiol. 54 (2013) 1278–1288. Crossref

[23]. M. Nafisi, M. Stranne, L. Zhang, J. van Kan, Y. Sakuragi, Mol. Plant Microbe Interact. 27 (8) (2014) 781–792. Crossref

[24]. B. Asselbergh, K. Curvers, S.C. Franca, K. Audenaert, M. Vuylsteke, F. Van Breusegem, M. Hofte, Plant Physiol. 144 (2007) 1863–1877. Crossref

[25]. M. Laskowski, S. Biller, K. Stanley, T. Kajstura, R. Prusty, Plant Cell Physiol. 47 (2006) 788– 792. Crossref

[26]. F. Passardi, C. Penel, C. Dunand, Trends Plant Sci. 9 (2004) 534–540. Crossref

[27]. F. Limam, K. Chahed, N. Ouelhazi, R. Ghrir, L. Ouelhazi, Phytochemistry 49 (1998) 1219–1225. Crossref

[28]. S. Paque, G. Mouille, L. Grandont, D. Alabadi, C. Gaertner, A. Goyallon, P. Muller, C. Primard- Brisset, R. Sormani, M.A. Blazquez, C. Perrot- Rechenmann, Plant Cell 26 (2014) 280–295. Crossref

[29]. S. Abuqamar, S. Ajeb, A. Sham, R. Iranti, Mol. Plant. Pathol. 14 (2013) 813–827. Crossref
Published
2017-09-15
How to Cite
[1]
N. Bishimbayeva, A. Murtazina, and G. McDougall, “Influence of Phytohormones on Monosaccharide Composition of Polysaccharides from Wheat Suspension Culture”, Eurasian Chem. Tech. J., vol. 19, no. 3, pp. 231-237, Sep. 2017.
Section
Articles