Oxidation of a Mixture of Polyaromatic Hydrocarbons by a Mixed Culture of Hydrocarbon-Oxidizing Microorganisms

D. A. Filatov; M. A. Kopytov; V. S. Ovsyannikova; E. A. Elchaninova

doi:10.18321/ectj1034

Authors

D. A. Filatov Institute of Petroleum Chemistry SB RAS, 4, Academichesky Ave., Tomsk, Russia
M. A. Kopytov Institute of Petroleum Chemistry SB RAS, 4, Academichesky Ave., Tomsk, Russia
V. S. Ovsyannikova Institute of Petroleum Chemistry SB RAS, 4, Academichesky Ave., Tomsk, Russia
E. A. Elchaninova Institute of Petroleum Chemistry SB RAS, 4, Academichesky Ave., Tomsk, Russia; National Research Tomsk Polytechnic University, 30, Lenin Ave., Tomsk, Russia

DOI:

https://doi.org/10.18321/ectj1034

Keywords:

polyaromatic hydrocarbons, biochemical oxidation, hydrocarbon-oxidizing microorganisms

Abstract

The possibility of biochemical oxidation of polyaromatic hydrocarbon mixtures (PAHs) by the mixed culture of hydrocarbon-oxidizing microorganisms (HOM) in a liquid medium and soil was investigated. The mixed HOM culture was represented by Pseudomonas stutzeri, Pseudomonas putida, Bacillus cereus, and Arthrobacter globiformis genera. It was shown that during HOM cultivation of the microorganisms under study in the liquid medium their number increases from 0.25·10⁴ to 11·10⁸ CFU/ml, which is accompanied by an increase in their oxygenase activity. All PAHs identified were subjected to oxidation from 11.3 to 100%. The results of experiments on biodegradation of PAHs under natural conditions have shown that for 60 days the total utilization of oil products in soils was on the average 65% of the initial contamination. This suggests the prospects for the use of the mixed HOM culture under study for effective biodegradation of PAHs polluting soil and waste waters.

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