Possibility of Using Modified Polyamide Membranes for Virus Concentration for the Purposes of Sanitary-Virological Analysis of Water

Authors

  • Yu. A. Fedotov Technofilter Research-and-Manufacturing Enterprise Ltd, 77 B.Nizhegorodskaya St., Vladimir, 600016, Russia
  • A. V. Tarasov Technofilter Research-and-Manufacturing Enterprise Ltd, 77 B.Nizhegorodskaya St., Vladimir, 600016, Russia
  • Yu. A. Rakhmanin A.N. Sysin Research Institute of Human Ecology and Environmental Hygiene, 10/15, Stroyeniye 1, Pogodinskaya St., Moscow, 119992, Russia
  • A. E. Nedachin Vladimir State University named after Alexander Grigoryevich and Nikolai Grigoryevich Stoletovs (VlGU), 87, Gorky Street, Vladimir, 600000, Russia
  • S. A. Lepeshin Technofilter Research-and-Manufacturing Enterprise Ltd, 77 B.Nizhegorodskaya St., Vladimir, 600016, Russia
  • A. I. Fedotova Technofilter Research-and-Manufacturing Enterprise Ltd, 77 B.Nizhegorodskaya St., Vladimir, 600016, Russia
  • Yu. T. Panov Vladimir State University named after Alexander Grigoryevich and Nikolai Grigoryevich Stoletovs (VlGU), 87, Gorky Street, Vladimir, 600000, Russia

DOI:

https://doi.org/10.18321/ectj119

Abstract

The results of investigations of modified polyamide microfiltration membranes application in the concentration of viruses and bacteriophages during sanitary virological monitoring of water bodies are presented in this paper. The analysis of developments in the control of viral contamination of different origins water bodies is given. Viruses presented in the water in small amounts can cause disease. It is shown that an important step is viral concentration from large amounts of water for its identification. The possibility of positively charged microfiltration membranes application in the concentration of viruses is established. A method of polyamide microfiltration membranes modification for increasing the positive charge on their surface is developed. To estimate the retention capacity of membranes the methods of measuring the sorption properties of the membranes using the model dye bromophenyl blue is developed. A membrane filter module with tangentially-radial mode of fluid movement was used. Studies carried out in specialized organizations revealed that the efficiency of bacteriophage MS-2 sorption on the modified polyamide membrane at different loads was 100%. It was found that the use of membrane filtration module not only improves the detention of microorganisms, but also allows improving the elution stage. The laboratory results were confirmed by tests using natural waters.

References

1. Haas, C.N., Rose, J.B., Gerba, C., and Regli, S., Risk Anal. 13:545 (1993).

2. Daisuke, S., You, U., and Tatsuo, O., Bull. Soc. Sea Water Sci. (Japan) 60(4):229 (2006).

3. Gerba, C.P., Wallis, C., and Melnick, J.L., Environ. Sci. Technol. 9:1122 (1975).

4. Gerba, C.P., Farrah, S.R., Goyal, S.M., Wallis, C., and Melnick, J.L., Appl. Environ. Microbiol. 35:540 (1978).

5. Liang, J.L., Dziuban, E.J., Craun, G.F., Hill, V., Moore, M.R., Gelting, R.J., Calderon, R. L., Beach, M.J., and Roy, S.L., MMWR Surveill Summ. Center for Disease Control and Prevention. 55(SS12):31 (2006).

6. Farrah, S.R., Gerba, C.P., Wallis, C., and Melnick, J.L., Appl. Environ. Microbiol. 31:221 (1976).

7. Gerba, C.P. Adv. Appl. Microbiol. 30:33 (1984).

8. L. Coin, in G. Berg (ed.) Transmission of Viruses by the Water Route, Interscience, London, 1967, p. 367.

9. Polaczyk, A.L., Narayanan, J., Cromeans, T.L., Hahn, D., Roberts, J.M., Amburgey, J.E., and Hill, V.R., J. Microbiol. Methods 73:92 (2008).

10. Lambertini, E., Spencer, S.K., Bertz, P.D., Loge, F.J., Kieke, B.A., and Borchardt, M. D., Appl. Environ. Microbiol. 74:290 (2008).

11. Sobsey, M. D., Moore, R. S., and Glass, J. S., J. Am. Water Works Assoc. 73:542 (1981).

12. Rebrikov, D.V., Samatov, G.A., Trofimov, D.Y., Semjonov, P.A., Savilova, A.M., Kofiadi, I.A., and Abramov D.D., PCR in the “real time”. BINOM, Moscow, Russia, 2009, 223 p.

13. Sanitary-virological control of water bodies. Methodological guidelines (MUK) 4.2.2029- 05. Moscow, 2006.

14. ICR Microbial Laboratory Manual US EPA/600/R-95/178. 1996.

15. Normalisation Francaise XP T 90-451. Essais des eaux. Recherche des Enterovirus. 1996.

16. Tarasov, A.V., and Fedotov, Y.A., A method for production of a microfiltration positively charged membrane. Patent RF No 2286842.

17. Stumpe, M., and Werner, U., Filtrieren und Separieren, 6(2):76 (1992).

18. Wallis, C., Henderson, M., and Melnick, J.L., Appl. Microbiol. 23:476 (1972).

19. Tarasov, A.V., Fedotov, Y.A., and Lepeshin, S.A., Perspektivnye materialy, 11:486 (2011).

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Published

2012-09-28

How to Cite

Fedotov, Y. A., Tarasov, A. V., Rakhmanin, Y. A., Nedachin, A. E., Lepeshin, S. A., Fedotova, A. I., & Panov, Y. T. (2012). Possibility of Using Modified Polyamide Membranes for Virus Concentration for the Purposes of Sanitary-Virological Analysis of Water. Eurasian Chemico-Technological Journal, 14(3), 233–242. https://doi.org/10.18321/ectj119

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