Phosphate Solubilization Potentials of Acinetobacter Strains and their Relations with Soil Properties

Authors

  • M. Ogut Selcuk University, Cumra Meslek Yuksekokulu, Cumra-Konya, Turkey
  • F. Er Selcuk University, Cumra Meslek Yuksekokulu, Cumra-Konya, Turkey
  • N. Kandemir Gaziosmanpasa University, Agricultural Faculty, Field Crops Department, Tokat, Turkey

DOI:

https://doi.org/10.18321/ectj50

Abstract

Phosphate solubilizing bacteria can be used as soil or seed inoculum to increase soil phosphorus (P)
availability for agricultural purposes. There is also a possibility of using these microorganisms to
biotechnologically dissolve phosphate ores for the production of phosphorus fertilizers. Twenty-one soil
samples were collected along a highway in Turkey to isolate phosphate solubilizing bacteria. A total of 20
phosphate solubilizers were isolated from the rhizosphere of wheat and maize grown in the pots, which
contained the collected soil samples. The isolates were distributed among the genera, Acinetobacter (7),
Pseudomonas (7), Enterobacter (2), Enterococcus (1), Escherichia (1), Photorhabdus (1), and Bacillus
(1) as determined by the 16S rDNA gene sequence analysis. Since the Acinetobacter species were most
effective in Pikovskaya’s agar, which contained tricalcium phosphate for the sole P-source, they were
further experimented for the phosphate solubilization in batch cultures. The mean phosphorus dissolved
in 5 day incubation ranged between 167 and 1022 ppm P. The initial pH of 7.8 dropped below 4.7 in six
isolates with a gluconic acid production in the concentrations ranging between 27.5 and 37.5 mM.
Acinetobacter isolates have some potential as an inoculum both for soil and biotechnological Psolubilization.

References

1. Adamowicz M., Conway T., Nickerson K.W. 1991. Nutritional complementation of oxidative glucose metabolism in Esceherichia coli via pyrroloquinoline quinone-dependent glucose dehydrogenase and the EntnerDoudoroff pathway. Appl. Environ. Microbiol. 57: 2012-2015.

2. Babu-Khan, S., T.C. Yeo, W.L. Martin, M. Duron, R.D. Rogers Goldstein AH. 1995. Cloning of a mineral phosphate-solubilizing gene from Pseudomonas cepacia. Appl. Environ. Microbiol. 61: 972-978.

3. Banik, S., Dey, B.K. 1982. Available phosphate content of an alluvial soil is influenced by inoculation of some phosphate solubilizing microorganisms. Plant Soil 69: 353-364.

4. Chabot, R., Beauchamp, C.J., Kloepper, J.W., Antoun, H. 1998. Effect of phosphorus on root colonization and growth promotion of maize by bioluminescent mutants of phosphatesolubilizing Rhizobium leguminosarum biovar phaseoli. Soil Biol. Biochem. 12: 1615-1618.

5. Fliege R., Tong S., Shibata A., Nickerson K.W., Conway T. 1992. The Entner-Doudoroff pathway in Escherichia coli is induced for oxidative glucose metabolism via pyrroloquinoline quinine-dependent glucose dehydrogenase. Appl. Environ. Microbiol. 58: 3826-3829.

6. Gaind, S., Gaur, A.C. 2002. Impact of fly ash and phosphate solubilizing bacteria on soybean productivity. Bioresource Technology 85: 313-315.

7. Goldstein, A.H., Braverman, K., Osorio, N. 1999. Evidence for mutualism between a plant growing in a phosphate-limited desert environment and a mineral phosphate solubilizing (MPS) rhizobacterium. FEMS
Microbiol. Ecol. 30: 295-300.

8. Goldstein, A.H., Rogers R.D., Mead G. 1993. Separating phosphate from ores via bioprocessing. Nature/Biotechnol. 11: 1250- 1254.

9. Hommes R.W.J., Postma P.W., Neijssel O.M., Tempest D.W., Dokter P., Duine J.A. 1984. Evidence of a quinoprotein glucose dehydrogenase apoenzyme in several strains of Escherichia coli. FEMS Microbiol. Lett. 24:329-333.

10. Illmer, P., Schinner, F. 1992. Solubilization of inorganic phosphates by microorganisms isolated from forest soil. Soil Biol. Biochem. 24: 389-395.

11. Kim, K.Y., Jordan, D., Krishnan, H.B. 1997. Rahnella aquatilis, a bacterium isolated from soybean rhizosphere, can solubilize hydroxyapatite. FEMS Microbiol. Lett. 153:273-277.

12. Kucey, R.M.N. 1987. Increased phosphorus uptake by wheat and field beans inoculated with a phosphorus-solubilizing Penicillium bilaji strain and vesicular-arbuscular mycorrhizal fungi. Appl. Environ. Microbiol. 53: 2699-2703.

13. Kumar, V., Narula, N. 1999. Solubilization of inorganic phosphates and growth emergence of wheat as affected by Azotobacter chroococcum mutants. Biol. Fertil. Soils 28: 301-305.

14. Liu S., Lee L., Tai C., Hung C., Chang Y., Wolfram J.H., Rogers R., Goldstein A.H. 1992. Cloning of an Erwinia herbicola gene necessary for gluconic acid production and enhanced mineral phosphate solubilization in E. coli HB101: Nucleotide sequence and probable involvement in biosynthesis of the co-enzyme pyrroloquinnoline quinone. J. Bacteriol. 174:5814-5819.

15. Narula, N., Kumar, V., Behl, R.K., Deubel, A., Gransee, A., Merbach, W. 2000. Effect of Psolubilizing Azotobacter chroococcum on N, P, K uptake in P-responsive wheat genotypes grown under greenhouse conditions. J. Plant Nutr. Soil Sci. 163: 393-398.

16. Nautiyal, C.S., Bhadauria, S., Kumar, P., Lal, H., Mondal, R., Verma, D. 2000. Stress induced phosphate solubilization in bacteria isolated from alkaline soils. FEMS Microbiol. Lett. 182: 291-296.

17. Pal, S.S. 1998. Interactions of an acid tolerant strain of phosphate solubilizing bacterial with a few acid tolerant crops. Plant Soil 198: 169-177.

18. Peix, A., Mateos, P.F., Rodriguez-Barrueco, C., Martinez-Molina, E., Velazque, E. 2001. Growth promotion of common bean (Phaseolus vulgaris L.) by a strain of Burkholderia cepacia under growth chamber conditions. Soil Biol. Biochem. 33: 1927-1935.

19. Peix, A., Rivas-Boyero, A.A., Mateos, P.F., Rodriguez-Barrueco, C., Martinez-Molina, E., Velazquez, E. 2001. Growth promotion of chickpea and barley by a phosphate solubilizing strain of Mesorhizobium
mediterraneum under growth chamber conditions. 33: 103-110.

20. Reyes, I., Bernier, L., Simard, R.R., Tanguay, P., Antoun, H. 1999. Characteristics of phosphate solubilization by an isolate of tropical Penicillium rugulosum and two UVinduced mutants. FEMS Microbiol. Ecol. 28:291-295.

21. Rodriguez, H., Gonzalez, T., Goire, I., Bashan, Y. 2004. Gluconic acid production and phosphate solubilization by the plant growthpromoting bacterium Azospirillum spp. Naturwissenschaften 91: 552-555.

22. Sundara, B., Natrajan, V., Hari, K. 2002. Influence of phosphorus solubilizing bacteria on the changes in soil available phosphorus and sugarcane and sugar yields. Field Crops Res. 77: 43-49.

23. Toro, M., Azcon, R., Barea, J. 1997. Improvement of arbuscular mycorrhiza development by inoculation of soil with phosphate-solubilizing rhizobacteria to improve rock phosphate bioavailability (32P) and nutrient cycling. Appl. Environ. Microbiol. 63: 4408-4412.

24. Van Kleef M.A.G., Duine J.A. 1989. Factors relevant in bacterial pyrroloquinoline quinine production. Appl. Environ. Microbiol. 55: 1209-1213.

Downloads

Published

2010-11-15

How to Cite

Ogut, M., Er, F., & Kandemir, N. (2010). Phosphate Solubilization Potentials of Acinetobacter Strains and their Relations with Soil Properties. Eurasian Chemico-Technological Journal, 12(3-4), 231–239. https://doi.org/10.18321/ectj50

Issue

Vol. 12 No. 3-4 (2010)

Section

Articles

License

You are free to: Share — copy and redistribute the material in any medium or format. Adapt — remix, transform, and build upon the material for any purpose, even commercially.

Eurasian Chemico-Technological Journal applies a Creative Commons Attribution 4.0 International License to articles and other works we publish.

Subject to the acceptance of the Article for publication in the Eurasian Chemico-Technological Journal, the Author(s) agrees to grant Eurasian Chemico-Technological Journal permission to publish the unpublished and original Article and all associated supplemental material under the Creative Commons Attribution 4.0 International license (CC BY 4.0).

Further distribution of this work must maintain attribution to the author(s) and the published article’s title, journal citation, and DOI.