Carbon Nanosorbent for Purification Different Biomolecules

Abstract

The article presents the results of physico-chemical studies on the development of nanostructured carbon materials from domestic raw materials. Were obtained and tested micro-mesoporous carbon sorbents for molecular-sieve chromatography of markers and investigated the applicability of carbon sorbents for the separation of protein-lipid complex, and plant bio-stimulator. Carbon sorbents have well-developed porous structure but their disadvantage is the weak mechanical strength. Recently it was shown that some carbon nanostructures have enormous strength. Thus arose the need to give the nano structured elements to carbon sorbent. Creating carbon sorbents containing nanocarbon structure was the aim of our study, as these by sorbents will be very useful for large-scale purification of biomolecules. The new carbon nanosorbent was prepared by carbonization of the stones of abricot seeds. The physico - chemical characteristics of nanostructured carbon sorbent was investigated by modern methods like scanning electrone microscope and infra red spectrophotometry. Based on the goal, nano-carbon materials in the laboratory of the Institute of Combustion Problems, Al-Farabi Kazakh National University synthesized nanostructured carbon sorbents – ‘Nanokarbosorb’ type for chromatography of biomolecules. It should be noted that the nanostructured elements of the frame attached to an unusually high mechanical strength to nanokarbosorb. Because of this, this sorbent can withstand high fluid pressure at work and has high durability, therefore, it can be used over the years. The sorbent has a very large porosity and large internal surface and, accordingly, a large capacity and has no parasitic sorption. Studies have shown that "Nanokarbosorb" suitable for purification of a powerful biostimulator plants. In this regard, of great interest represents nanostructured carbon sorbents with improved chromatography characteristics. Carbon sorbents known to mankind over thousands of years. They arewidely used for purification of alcohol and other solutions.
They are mechanically very weak and quickly attacked by fungi and bacteria. In this reason they are unconvenient large scale purification biomolecules. However, further improvement of chromatographic sorbents is impossible without the use of ideas and techniques of nanotechnology.